WebMar 2, 2024 · Overexpression of AMPKα2 prevents the phenylephedrine (PE)-induced impairment of mitophagy in CMs. A. HL-1 mouse CMs were infected with an adenovirus encoding AMPKα2 and then subjected to PE (50 μmol/L) stimulation, followed by the induction of mitophagy. Mitophagy induction was performed by treatment with 20 … WebMitophagy serves as a specific elimination system that dysfunctional mitochondria caused by oxidative stress and DNA damage are sequestered into autophagosome, fused to lysosome and degraded by digestion. ...
Flow Cytometry-Based Assessment of Mitophagy Using …
WebDec 26, 2024 · Mitophagy is a selective autophagy process that specifically removes damaged mitochondria via general autophagy. The two major recessive Parkinson's disease genes PINK1 and Parkin play essential roles in mitophagy initiation, increasing the interest in mitophagy in both basic and translational research over the past 10 years. WebMitophagy is a selective autophagy process that specifically removes damaged mitochondria via general autophagy. The two major recessive Parkinson's disease genes PINK1 and Parkin play essential... moderate intensity bpm
Sensitive Measurement Mitophagy Flow Cytometry Using Ph …
WebNov 11, 2024 · Interestingly, it has been documented that hypoxia-induced mitophagy is mediated by various factors in different cell types . Although we have found that BNIP3-dependent mitophagy is associated with the neuroprotective effects of pinocembrin, it is still vague whether it plays a dominant role in the inhibition of IH-induced … WebMay 2, 2024 · Mitophagy in the absence of Ubl-phosphorylation. Mitophagy was detected by fluorescence-activated cell sorting (FACS) of untreated and CCCP-treated (20 μM for 4 h) U2OS cells containing mitochondrially targeted mKeima (mt-Keima) and transiently expressing GFP-parkin. Webhow to design and plan mitophagy experiments for FACS and how to perform FACS analysis to obtain the final data. BASIC PROTOCOL 1 GENERATION OF STABLE … innisfree time science spot treatment