Fitc labeled collagen
WebMentioning: 44 - We evaluated alkaline-solubilized collagen, hyaluronic acid (HA), and a substance formed from mixing both materials as vitreous substitutes in the rabbit. Fluorescein isothiocyanate (FITC)-labeled collagen (2%), fluorescein-labeled HA (FL-HA, 1%), or the mixture was injected into the rabbit vitreous after vitrectomy. After 3 … WebMar 1, 2024 · (a) Fluorescence microscopy analysis showing the colocalization of FITC-labeled CNA35 (green; upon incubation of tissue sections) with collagen fibers of type I, III, and IV (Col I, Col II, and Col III, respectively; all in red) on renal tissue sections from healthy mice and mice with unilateral ureteral obstruction. Nuclei are counterstained ...
Fitc labeled collagen
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WebFITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. ... WebFeb 1, 2002 · In the current studies, we utilized a fluorescein-isothiocyanate-labeled substrate to develop novel zymographic and reverse … Real-time zymography and reverse zymography: a method for detecting activities of matrix metalloproteinases and their inhibitors using FITC-labeled collagen and casein as substrates Anal Biochem. …
WebDec 18, 2024 · FITC-labeled collagen was prepared as follows: a small amount of FITC (Dojindo Laboratories, Kumamoto, Japan) was added into the 1.0 w% atelocollagen solution (20 mL) for the reaction of atelocollagen and FITC (room temperature, 1 h). The reaction mixture was dialyzed by a cellulose semipermeable membrane (MWCO 8000–14 000 … WebType I collagen is the major collagen of tendon and bone, but it is widely distributed in other tissues, such as skin, cornea, tooth dentin, heart valve, lung, liver, fascia and scar …
WebDo you offer FITC labeled primers? IDT offers a 6-FAM fluorophore modification instead of the traditional FITC fluorophore modification. FITC and FAM are both derivatives of … WebCollagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and …
WebMar 29, 2024 · Collagen was largely distributed in tumors compared to normal tissues. (B, C) FITC-labeled SIRPαFc and CBD–SIRPαFc distributed in tumor, and main organs at 2 h after intraperitoneal injection were detected, when more CBD–SIRPαFc accumulated in tumor than SIRPαFc (n = 3) (B).
WebNov 1, 2003 · (B) Lack of FITC release from FITC-labeled collagen matrices by T cells, but not by HT-1080/MT1 cells (positive control). ConA-stimulated CD4 + T cells or HT-1080/MT1 cells were cultured in 3D collagen lattices containing 2% FITC-collagen in the absence or presence of protease inhibitor cocktail for 40 hours. Data show the means + SDs for 3 ... images of sharing with othersWebOct 15, 2015 · Collagen was internalized and degraded by hMSCs in both 2D and 3D models. Figure 1a-c showed the presence of fluorescence (Alexa 488)-labeled collagen inside the human MSCs as soon as 1 h after incubation with serum-free culture medium containing collagen. The internalized collagen was degraded as shown by the presence … images of shark tattoosWebCollagen Type I Fitc, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more list of blood infection namesWebMar 28, 2024 · The excessive Cy5-CpG 1826 was removed by washing with PBS for 3 times before the addition of FITC labeled PDA NPs (50 μg/mL) or not. After incubation (24 h), the cells were stained with Lyso- Tracker Red (Beyotime, China) and DAPI for observation by confocal microscope (Leica SP8, Germany). images of sharing the gospel of jesusWebApr 5, 1988 · Interaction of fibronectin and its gelatin-binding domains with fluorescent-labeled chains of type I collagen. Author links open overlay panel K C Ingham, S A Brew, B S Isaacs. Show more. ... (Eds.), Collagen in Health and Disease, Churchill Livingstone, Edinburgh, Scotland (1982), pp. 198-205. View in Scopus Google Scholar. 19. J. Engel, … images of shannon irelandWebNov 18, 2024 · The next day a 50 μg/ml stock of FITC-Col was prepared by mixing 500 μL of collagen type 1-FITC (1 mg/mL Chondrex cat# 4001) with 9.5 mL media, incubating 20 … images of shark vacuum cleanersWebFeb 26, 2024 · Collagen (Type I), FITC conjugated Water Insoluble - 5 mg. Together with the water-soluble collagen (Type I) conjugate (Cat# AS-85111), this water-insoluble … images of sharing essential oils