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Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 ...

A previously described31 environmental strain of P. aeruginosa was used as a template to amplify the four cas genes of the Type I-C CRISPR–Cas system genes (cas3, cas5, cas7 and cas8). The genes were cloned into … Zobraziť viac Transformations of P. aeruginosa, E. coli, P. syringae and K. pneumoniae strains were conducted using standard electroporation … Zobraziť viac PAO1IC was grown overnight at 37 °C in LB media. Then 150 μl of overnight culture was added to 4 ml of 0.7% LB top agar and spread on 1.5% LB agar plates supplemented … Zobraziť viac The isogenic DMS3macrIIA4 and acrIC1 phages were constructed using previously described methods60. A recombination cassette, pJZ01, was constructed with homology to the … Zobraziť viac WebpHERD20T铜绿假单胞菌阿拉伯糖诱导表达载体接合转移质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 pHERD20T, an arabinose-inducible expression vector. pHERD20T is a … spine issues lower back

CRISPRi-Mediated Gene Suppression Reveals Putative Reverse ...

Web1. okt 2024 · PAO1 harbouring pHERD20T‐dCas9 or pHERD20T‐dCas9‐prtR were used in the tests. (d) Plating efficiencies of 5 × 10 8 /ml bacterial cells (PAO1 harbouring … Webpet15b,pet-15b载体价格800元，载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见下文，抗性为Ampicillin，大小为5708bp，测序引物为T7和T7t，含有N-His标签和N … WebThe key point is that the dcas9 gene is located downstream of the P BAD promoter. pHERD20T is an arabinose-inducible vector based on pUCP20T that supports replication … spine itching