2024 Tph2-cre mice

Tph2-cre mice

Author: rwuj

August undefined, 2024

Splet01. mar. 2024 · Mice (10-12weeks old) expressing Cre-recombinase under the Tph2 specific promoter and with Exon V of Tph2 flanked by loxP sites were injected with … Splet06. avg. 2014 · Here, we report the generation of a Pet1210-Cre transgenic mouse line in which the Cre recombinase is expressed under the control of a 210 kb fragment from the Pet1 genetic locus to ensure a reliable and faithful control of …

Free-floating Immunostaining of Mouse Brains - PMC

SpletTime courses of REM sleep (top) or NREM sleep (bottom) in GAD2-Cre mice after 593-nm or 470-nm laser stimulation of axonal terminals in the dDpMe or ChR2-mCherry-expressing neurons in the SLD/MPB ( c ), LH ( h ) initiated from REM sleep. Splet02. mar. 2024 · Here, we have generated mutations for the gene tryptophan hydroxylase 2 ( Tph2) in rodents and non-human primates. It is required for synthesizing 5-hydroxytryptamine (5-HT) in the brain. 44 , 45 , 46 , 47 , 48 These mutants allowed us to discover a conserved role for 5-HT in affiliation. tots who travel

Tie2-Cre transgenic mice: a new model for endothelial cell-lineage ...

SpletMethods: SOD1(G37R) mice expressing a conditional allele of an ALS-linked SOD1 mutation were crossed with Tph2-Cre mice expressing Cre in serotonergic neurons. Measurement … SpletWe examined the novel interaction between BDNF (Val66Met) and TPH2 (rs4570625) polymorphisms and their influence on behavioral inhibition of negative emotion in two independent investigations of ... Splet01. jan. 2024 · Here we systematically investigated the effects of TPH2 on energy homeostasis in adult male and female mice. We found that the DRN harbors a similar … potholderz sample

Orexin receptors 1 and 2 in serotonergic neurons ... - Nature

Modulation of depression-related behaviors by adiponectin …

Splet014554 B6.129(Cg)- Slc6a4 tm1(cre)Xz /J The SERT-cre (SERT cre ) "knock-in" allele has a nuclear-localized Cre recombinase inserted just upstream of the first coding ATG of the … SpletBoth mutant mouse strains displayed, at 3 mo of age, the same low-bone-mass phenotype that was observed in either Tph2 −/ ... Galactosidase staining was performed on the tissues obtained from the Sf1-Cre/rosa26 mice following standard procedures. Briefly, tissue samples were dissected after intracardial perfusion with ice-cold 4% ... tots westfield stratfordSplet02. sep. 2024 · Orexin-Cre mice were created and the expression pattern of Cre was analyzed by crossing Orexin-Cre transgenic mice and ... c-Fos (c-Fos, 316921-C4), TPH2 … tots with that

"Splet11. apr. 2002 · Generation of Thy1-Cre Transgenic Mice A 1.1-kb Cre cDNA containing a nuclear localization signal was amplified by the polymerase chain reaction (PCR) with Xho I-containing primers; forward primer 5′-TTACCCTCGAGGAGAACCACCATGG-3′ and reverse primer 5′-ATACCCTCGAGGGATGGCTAATCGC-3′. " - Tph2-cre mice

Tph2-cre mice

Modulation of depression-related behaviors by adiponectin …

SpletCKO 2012.10.13. nestin Cre mice, cx43 fl/fl라는건, 기존의 낙아웃 마우스는 마우스 몸 전체에서 원하는 gene을 없앤경우인데, tissue specific하게 knockout 시킨 경우를 conditional knockout이라고 부릅니다. tissue specific하게 knockout 시키기 위해서는 원하는 tissue에만 발현하는 gene의 ... Splet21. avg. 2024 · Genetic ablation of tph2 in the pons/midbrain raphe nuclei (groups B5–B9) of adult mice was shown to cause hyperactivity and eliminate siestas (Whitney et al., …

Did you know?

Splet05. dec. 2024 · By crossing the new Tph1 floxed mice with tissue-specific Cre mice, we demonstrate highly efficient reduction in 5-HT synthesis in peripheral tissues. These … SpletTph2 KO mice (Tph2 -/mice) are characterized by depletion of serotonin synthesis within the brain 302 ( Figure 13 and Figure 12) but the peripheral serotonin system remains unaffected. ......

Splet008601 B6.Cg- 7630403G23Rik Tg(Th-cre)1Tmd /J TH-Cre transgenic mice have the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to … Splet04. apr. 2011 · A role for 5-hydroxytryptamine (5-HT) was demonstrated by the phenotype of mice unable to synthesize 5-HT in the brain when lacking tryptophan hydroxylase 2 (Tph2). 5-hydroxytryptophan (5-HTP) injection rescued the phenotype of adult Tph2knockout mice within 35 minutes.

Splet19. mar. 2024 · 该研究构建了一种新的诱导型Cre重组酶，可通过自我剪切将诱导型CreER转变成持续活化的Cre，实现时间可控的高效遗传操作。这种新型Cre重组酶结合了诱导型CreER和持续活化的Cre重组酶的优点，既提高了重组效率又具有时间可控性，弥补了传统CreER和Cre重组酶介导的遗传操作技术的缺陷，突破了长期阻碍基因功能研究的瓶颈， … SpletGemPharmatech is a leading provider of genetically engineered mouse models (GEMMs) and services to global preclinical R&D communities. We specialize in developing animal models using cutting-edge gene-editing technologies with a large collection of KO/KO (conditional knockout/knockout) mice, humanized mice, immunodeficient mice, and …

Splet30. jul. 2008 · Tph2 was inactivated in mice using the Cre/LoxP strategy resulting in viable Tph2 null mutants. The preliminary results indicate that raphe serotonergic neurons in mice with a targeted disruption of Tph2 are completely devoid of 5-HT immunoreactivity, whereas no alterations in morphology and fiber distribution appear to occur.

Spletcre工具鼠 3-8w 雌/雄. 服务名称：江苏艾菱菲生物科技有限公司技术资料：0 篇. cre工具鼠提供定制服务，部分工具鼠有常规现货。. 欢迎咨询购买. 江苏艾菱菲生物科技有限公司. 2年江苏. 查找相似产品查找同商家产品. ￥2500. 加入对比查看详情在线咨询. tots wr jtohSplet04. mar. 2024 · 首先最基础的小鼠，C57BL/6，它是一种常见的近交品系实验鼠，我们常听到的 “C57”或者“B6”都是它。因为该小鼠品系已经完成了基因组测序，且对许多肿瘤不易发，但能够发生饮食诱导性肥胖和动脉粥样硬化等。声明：该文观点仅代表作者本人，搜狐号系信息发布平台，搜狐仅提供信息存储空间服务。首赞阅读 () 0 人参与， 0 条评论 pot holder with pocket tutorialSplet09. maj 2024 · 今天看到一段对Cre+/-，flox/flox的描述，受益匪浅，摘录下来，以备后用。 Cre+/-，flox/flox表示携带1个Cre基因的条件剔除小鼠 Cre ... potholder yarnSplet21. feb. 2024 · 一、品系介绍： Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。 Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发 … pot holding rackSpletThese Tek-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in endothelial cells during embryogenesis and adulthood. Cre recombinase activity is also … tots with thoughtsSpletSTOCK Tg(Tph2-cre)RH35Gsat/Mmucd. Stock Number: 036634-UCD. Citation ID: RRID:MMRRC_036634-UCD Copy RRID Copy Citation. Major Collection: ... If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing ... pot holdingSpletEnter the email address you signed up with and we'll email you a reset link. tots wine